Will a low 260/230 ratio effect results using MiSeq?

[rjoyons]

Hi Guys!

I am new to Illumina sequencing and I have a sample pretty much ready to go containing around 1ug of genomic DNA. I checked the quantity using agarose gel comparing the genomic band to a quantitative ladder and another quantitative marker. The 260/280 ratio is 1.8 but the 260/230 ratio is only 1.2

Will this be detrimental to a run with a MiSeq?

Thanks in advance!

[microgirl123]

If I was prepping your library, I would strongly urge you to clean it up more before submitting it. We've made a couple libraries with poor 260/230 ratios (<1.5) and gotten yields too low to load on the MiSeq.