Will a low 260/230 ratio effect results using MiSeq?

rjoyons

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Join Date: Jul 2013

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Will a low 260/230 ratio effect results using MiSeq?

08-28-2013, 05:53 AM

Hi Guys!

I am new to Illumina sequencing and I have a sample pretty much ready to go containing around 1ug of genomic DNA. I checked the quantity using agarose gel comparing the genomic band to a quantitative ladder and another quantitative marker. The 260/280 ratio is 1.8 but the 260/230 ratio is only 1.2

Will this be detrimental to a run with a MiSeq?

Thanks in advance!
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microgirl123

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Join Date: Jun 2012

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08-29-2013, 07:42 AM

If I was prepping your library, I would strongly urge you to clean it up more before submitting it. We've made a couple libraries with poor 260/230 ratios (<1.5) and gotten yields too low to load on the MiSeq.
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Will a low 260/230 ratio effect results using MiSeq?

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