Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • Pepe
    Member
    • Mar 2009
    • 30

    TopHat coverage.wig file wish list

    Hi,

    I wanted to add to the list of things to have into account for the next version of TopHat, in case the developers are around.
    I'm using Tophat v1.0.12 and Bowtie v0.11.3
    I noticed that the last line for each chrm in the coverage.wig file ends a position earlier. For example, the last lines for scaffold1 in my coverage.wig file are:

    scaffold1 4408480 4412926 0
    scaffold1 4412926 4413006 1
    scaffold1 4413006 4486297 0
    scaffold1 4486297 4486376 1

    I'm using 80 bp reads as you can see in the 2nd line pasted above (4413006-4412926=80), but the last line of the wig file shows a read of length 79 (4486376-4486297=79) (I've checked the SAM file and this read aligns in all 80 bases) I noticed that this happens on the last read/covered fragment of every chrm.

    Do I get a price for helping make Tophat even a greater software than it'salready? If so, I would like to make one more wish.
    All the chrms end on that last read/fragment aligned as opposed to have a last row with deph of coverage equal to 0 starting from the end of the last read/fragment and finishing at the end of the chrm.
    In the example above scaffold1 is 4553411 nt long. A nice way to finish the chrm would be:
    scaffold1 4486376 4553411 0

    Finishing like this this will make the wig file complete and accurate, and will make it useful for coverage calculations, etc.

    Thanks for developing a great software.
  • Xi Wang
    Senior Member
    • Oct 2009
    • 317

    #2
    I check my results. The same case.
    Xi Wang

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
      by SEQadmin2


      Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

      The systematic characterization of the human proteome has
      ...
      07-20-2026, 11:48 AM
    • SEQadmin2
      Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
      by SEQadmin2



      Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
      ...
      07-09-2026, 11:10 AM
    • SEQadmin2
      Cancer Drug Resistance: The Lingering Barrier to Rising Survival
      by SEQadmin2



      Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.

      There is no single reason why many patients don’t respond to treatment as expected. Cancer is...
      07-08-2026, 05:17 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 07-24-2026, 12:17 PM
    0 responses
    28 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-23-2026, 11:41 AM
    0 responses
    21 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-20-2026, 11:10 AM
    0 responses
    210 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-13-2026, 10:26 AM
    0 responses
    78 views
    0 reactions
    Last Post SEQadmin2  
    Working...