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  • pmiguel
    Senior Member
    • Aug 2008
    • 2328
    #31
    Originally posted by armins View Post
    Phillip,

    All I know is that gDNA fragments are present in some of my samples, based on the shape of the "inter-region" (I'm trying to attach one of the Bioanalyzer results). I failed to mention that due to the processing of my samples, gDNA is sheared into pieces, so it would show up on the Bioanalyzer graph.
    How did the genomic DNA get sheared?
    Is the hump between the 18S and 28S peaks supposed to be DNA?

    --
    Phillip

    Comment

    • armins
      Junior Member
      • Dec 2013
      • 3
      #32
      Originally posted by pmiguel View Post
      How did the genomic DNA get sheared?
      Is the hump between the 18S and 28S peaks supposed to be DNA?
      The lysate was passed through QIAshredder columns a couple of times which I believe breaks up the DNA. What that hump is? I am not positive, but according to Agilent in their case it is DNase-sensitive. Page 82 here:
      Access Denied
      https://www.chem.agilent.com/Library/applications/5991-3322EN.pdf

      Comment

      • Janan
        Junior Member
        • May 2016
        • 1
        #33
        DNase treatment

        Hello,

        I am using mirVana™ miRNA Isolation Kit, to isolate small RNA from animals blood. Do I have to do DNase treatment for it?

        Thanks,

        Janan

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