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  • Samarpana
    Member
    • Dec 2013
    • 16
    #1

    Illumina Truseq PCR enrichment pre-pooling issue

    Hi
    I am currently using Truseq kits. Just finished with sample prep. On gel, I could distinctively see one bright band but after gel extraction followed by PCR amplification, Bioanalyzer is showing 2 peaks - one between 200-500bp(desired) and other around 9bp-1kb!! The intensity of both are similar.
    Could it be a problem with PCR kit?
    I am really confused. Please suggest possible reason for this and a solution, if any.
    P.S. I diluted my DNA sample with crosslinked MQ for Bioanalyzer reading?
    Tags: illumina, pcr cleanup, truseq
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