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I have a collection of genomic contigs that I want to scaffold using a closely related species' genomic reference as the backbone. I just want to use the reference to orient and space the contigs, and then fill in gaps with Ns. What would be the best (and simplest) way to accomplish that?
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ABACUS was designed for ordering contigs relative to a reference, and with IMAGE you can fill the gaps with your reads. Both programs are a part of the PAGIT pipeline. In my experience, ABACUS is quite straightforward to use but IMAGE is very buggy; it will change the name of you input files, create lots of temporary files and fail without clear warnings. Also, I recall having to change some of the code with IMAGE to get it working correctly. -
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Scaffold_builder (http://www.scfbm.org/content/8/1/23) looks straight-forward and could be a good tool for that purpose. Haven't had the time to test it myself yet though.MSc Bioinformatics student at the Free University Berlin , GermanyComment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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