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Hello, I am new to this forum. I am writing this thread because I wanted to know what would be the best way to go about mapping RNA seq reads that I have to my reference gneome (hg 19 genome). I have 8 fastq files that I need to map. I am new to this mapping and need help.
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Basic guide: http://en.wikibooks.org/wiki/Next_Ge..._%28NGS%29/RNA
TopHat/Cufflinks: http://www.nature.com/nprot/journal/....2012.016.html
If you are a biologist wanting to learn (enough) UNIX to use some of the programs above then part I of this guide: http://korflab.ucdavis.edu/Unix_and_...rl_v3.1.1.html -
Thank you very much.Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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