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Old 01-27-2010, 01:20 PM   #3
Nils Homer
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Location: Boston, MA, USA

Join Date: Nov 2008
Posts: 1,285

I just looked at an old DH10B run (2008) and found that the the mapping rate (% of bases mapped) went from 55% to 70% but at the cost of an increased mismatch rate and identified color error rate (even at higher mapping qualities). They (ABI) are recommending to only use this with smaller genomes (less than 200Mb). I will still test it out with skepticism.
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