Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • variadevang
    Junior Member
    • Jun 2012
    • 7

    #1

    Conver\

    Hello All,
    NGS data from SRA is downloaded in form of .sra files which are highly compressed and save a lot of space. fastq-dump converts .sra to fastq. i am interested in something which converts fastq, back to sra, i learnt fastq-load does it, but cannot find any manual explaining how to. Does anyone know how to and can help?
  • gringer
    David Eccles (gringer)
    • May 2011
    • 845

    #2
    well, running the help from the command line will probably give you quite a lot of help:
    Code:
    $ fastq-load --help
    
    Usage:
            fastq-load [options] -r run.xml -e experiment.xml -o output-path
    
      -r|--run-xml                     path to run.xml describing input files 
      -e|--experiment                  path to experiment.xml 
      -o|--output-path                 target location 
    
    Options:
      -i|--input-path                  input files location, default '.' 
      -u|--input-unpacked              input files are unpacked 
    ... etc.
    The XML schemas for Run / Experiment can be found on the NCBI SRA website.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
      by SEQadmin2



      CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

      Despite this, “CRISPR helped turn genome editing from a specialized technique into
      ...
      07-31-2026, 11:01 AM
    • SEQadmin2
      Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
      by SEQadmin2


      Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

      The systematic characterization of the human proteome has
      ...
      07-20-2026, 11:48 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 08-06-2026, 07:41 AM
    0 responses
    23 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 08-03-2026, 10:13 AM
    0 responses
    37 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-31-2026, 02:55 AM
    0 responses
    43 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-24-2026, 12:17 PM
    0 responses
    26 views
    0 reactions
    Last Post SEQadmin2  
    Working...