HiSeq SBS & Cluster V4 kits.

[weigrc]

Hello all,

Just saw the V4 kit information released on Illumina website (http://www.illumina.com/products/hiseq-sbs-kit-v4.ilmn).

"......The new v4 reagents increase the number of clusters by 33% compared to the TruSeq SBS Kit v3".......

Does anyone know when the kits can be ordered regularly?!? Any has tried the V4 kit?!?





Thanks,
Wei

[GenoMax]

As the linked page says these reagents are for the "1Tb" upgrade which has not been officially released at this time (the title also indicates "pre-release").

Even if someone managed to order a V4 kit, they may not be able to use it until the associated hardware/software upgrades are in place on the HiSeq 2500/1500 (and a select few HiSeq 2000's).

[Baseless]

And the very best of it is that not all HiSeqs are updatable. They just told me our camera is too slow and they will not upgrade cameras.
Together with the fact they seem to plan to weed out the smaller centers by selling the more cost efficient HiSeq X only in packages of ten, I start reaching a point where I whish that they get more competition. FAST.

[Zuuhl]

In a sales pitch they state that all 2000s shipped in 2013 and all factory build 1500/2500 can have their firmware+software updated to run the v4 kits.

Right know I'm trying to figure out, what prevents a field upgraded 2500 from running v4?

I know that the new generation of cameras were added from SN 700895 and forward.
This began summer 2011.

Fluidics were upgraded on a lot of the 2000s late 2012/early 2013.

Most likely PCs were improved at a time as well?

Anyone who has been able to find more detailed information on this subject?


Just had confirmed, that the camera is the bottle neck on older HiSeqs.

[Baseless]

PCs were upgraded, I think some memory, but they got a more performant disk array for sure.

They say the cameras are too slow. But I am greatly confused that they do not offer to upgrade the early HiSeqs.

[GW_OK]

I know that the new generation of cameras were added from SN 700895 and forward.
This began summer 2011.

That's for v2 of the cameras. v3 cameras started at SN ~701405. You have to have a v3 camera to get the 1T upgrade.

[Zuuhl]

That's for v2 of the cameras. v3 cameras started at SN ~701405. You have to have a v3 camera to get the 1T upgrade.

Hmm? I might have to double check my answer from Illuminas techsupport then.

Perhaps they looked at the S/Ns cleared for new rapid kits and not the high output?

[maggielaw]

I found out this information from Illumina's HiSeq v4 System Upgrade Information Sheet.
Compatible systems for Cluster v4 kits:
HiSeq 2500 (SN# D00101 or higher)
HiSeq 1500 (SN# C00101 or higher)
HiSeq 2000 (SN# 7001403 or higher)
Field Upgraded HiSeq 2500 (SN# 7001403 or higher)
Field Upgraded HiSeq 1500 (SN# L179 or higher)

[GenoMax]

http://core-genomics.blogspot.com/2014/04/more-of-fizz-than-bang-hiseq-1tb-is-now.html