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  • bioenvisage
    Member
    • Oct 2009
    • 40

    #1

    velvet denovo

    Hi ..I was running velvetg with various parameters but for different coverege cutoff iam getting the different results...how to optimize the coverege cutoff ..


    thanks
  • francesco.vezzi
    Member
    • Jan 2009
    • 50

    #2
    In the manual there is a description of how generate an histogram that can help you in decide several parameters.
    In general the best way to proceed is make some test on a little data set. You keep the parameters that give the best result on the small data set and you use them on the whole data set

    Francesco

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    • Zigster
      Jeremy Leipzig
      • May 2009
      • 117

      #3
      you should also look at N50, read usage, and the count of 1k contigs

      the script in my sig might help you
      --
      Jeremy Leipzig
      Bioinformatics Programmer
      --
      My blog
      Twitter

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