I've finished my first metagenome assembly and would like to know if there is a good ORF finder I could try out before proceeding to functional annotation with CAMERA for example.
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Thank you, I also found out CAMERA has an ORF finder as well.Comment
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vanillasky...are you mining assemblies for specific genes/enzymes?
i'm trying to figure out a more automated way of picking out ORFs that relate to specific enzyme families.Comment
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Hi Lac302! Maybe you can clarify what you mean by mining assemblies? I am interested in annotating the contigs coming out of my assembled metagenomes. Ultimately, yes I would like to focus on those contigs containing genes related to a certain function.Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Yesterday, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
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