AMOS & BAMBUS program experience sharing and general user guide.

[Patrick]

Hi,

Anybody willing to share the experience of using AMOS & BAMBUS program?
Can give the general explanation about this two program and general user guide of these two program?
Thanks a lot for sharing and advise

[[email protected]]

HI Patrick,

Did you get any assistance about AMOS. I am at the same position when you did this post. Any help you can do?

[Patrick]

Hi,

I just subscribe with the AMOS help. I learning from there now. What problem you have facing now? Anything I can help you too? Maybe we face the same situation too.

[[email protected]]

i have installed AMOS but i dont know how to make relevant input formats from illumina reads and reference sequence.

[jjohnson]

Adnan,

AMOS is a pretty large suite of tools, what are you trying to do more specifically, maybe I can share some war stories if I know where to start.

[[email protected]]

Thanks johnson. I am actually intending to do comparative assembly of bacterial strain whose data is from illumina GA2 with 75bp of reads. The data was in scarf format and i converted it into standard fastq format. Now the thing is that AMOScmp-shortreads takes two inputs .1con and .afg. How to convert reference sequence to .1con? And which steps should i follow till final assembly, viewing and validation? Thanks

[[email protected]]

Also how i convert paired end reads in .afg format since nowhere on AMOS pages is mentioned about paired end reads conversion. I am gone mad in this, no help on the web and at AMOS-help.

[Kasycas]

Hi [email protected],

Did you find out about this reference file? I can't find much info anywhere about it!

Thanks,

Kasycas

[catfisher]

I got an error when I make files for Bambus, can anybody give help? Thanks,
The error is bellow:

for i in src doc ;do cd $i ; make all; cd .. ; done
make[1]: Entering directory `/home/ngs/SL/software/bambus-2.33/src'
for i in IO DotLib TIGR_Foundation_CC grommit ;do cd $i ; make all; cd .. ; done
make[2]: Entering directory `/home/ngs/SL/software/bambus-2.33/src/IO'
make[2]: Nothing to be done for `all'.
make[2]: Leaving directory `/home/ngs/SL/software/bambus-2.33/src/IO'
make[2]: Entering directory `/home/ngs/SL/software/bambus-2.33/src/DotLib'
make[2]: Nothing to be done for `all'.
make[2]: Leaving directory `/home/ngs/SL/software/bambus-2.33/src/DotLib'
make[2]: Entering directory `/home/ngs/SL/software/bambus-2.33/src/TIGR_Foundation_CC'
g++ -D_HAS_GETOPT -c -o ConfigFile.o ConfigFile.cc
ConfigFile.cc: In member function ‘void ConfigFile:arseConfig()’:
ConfigFile.cc:113: error: ‘strlen’ was not declared in this scope
make[2]: *** [ConfigFile.o] Error 1
make[2]: Leaving directory `/home/ngs/SL/software/bambus-2.33/src/TIGR_Foundation_CC'
make[2]: Entering directory `/home/ngs/SL/software/bambus-2.33/src/grommit'
g++ -I../TIGR_Foundation_CC/ -g -c grommit.cc
grommit.cc: In function ‘int main(int, char**)’:
grommit.cc:236: warning: format ‘%s’ expects type ‘char*’, but argument 3 has type ‘char (*)[1024]’
grommit.cc:317: error: ‘abort’ was not declared in this scope
make[2]: *** [grommit.o] Error 1
make[2]: Leaving directory `/home/ngs/SL/software/bambus-2.33/src/grommit'
make[1]: Leaving directory `/home/ngs/SL/software/bambus-2.33/src'
make[1]: Entering directory `/home/ngs/SL/software/bambus-2.33/doc'
make[1]: Nothing to be done for `all'.
make[1]: Leaving directory `/home/ngs/SL/software/bambus-2.33/doc'