Unconfigured Ad

Collapse
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • Simone78
    Senior Member
    • Oct 2010
    • 208
    #1

    Has anybody tried the Ovation Single Cell RNA-seq system?

    Hi,
    it would be interesting to hear how good it is...the kit costs about 5000 USD for 32 samples! In the FAQ sections they say the kit is designed for high-quality RNA (NOT whole cells, apparently...so why calling it "Single-Cell"?) and the amount of rRNA reads will be <25% (seriously? after paying all that money 1/4 of my library will be junk?).
    Besides, what are these nucleotide analogs that are incorporated during the first cDNA strand synthesis and later they are used for the fragmentation? Any idea? thanks!
    Tags: hexamer, nugen, single-cell sequencing, total rna
  • Nighthawkrao77
    Member
    • Jan 2015
    • 11
    #2
    Bumping this thread because I am curious as well.


    We tried 500 pg of good quality total RNA with this kit. It had horrible results - 60-90% Duplication rate, 30% rRNA fractions. Anyone else have this experience?

    Comment

    Previous template Next

    Latest Articles

    Collapse
    • GATTACAT
      Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
      by GATTACAT
      Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
      07-01-2026, 11:43 AM
    • SEQadmin2
      Nine Things a Sample Prep Scientist Thinks About Before Sequencing
      by SEQadmin2


      I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

      Here are nine questions we think about, in roughly the order they matter, before...
      06-18-2026, 07:11 AM
    View All

    ad_right_rmr

    Collapse

    News

    Collapse
    Topics Statistics Last Post
    High-Resolution Sequencing Exposes Hidden Toxoplasma Diversity by SEQadmin2
    Started by SEQadmin2, 07-02-2026, 11:08 AM
    0 responses
    24 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    07-02-2026, 11:08 AM  
    New AI Model Captures Long-Range Genomic Signals to Improve RNA Splice Site Prediction by SEQadmin2
    Started by SEQadmin2, 06-30-2026, 05:37 AM
    0 responses
    23 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    06-30-2026, 05:37 AM  
    Large-Scale Protein Screen Uncovers Hidden Regulators of Alternative Polyadenylation by SEQadmin2
    Started by SEQadmin2, 06-26-2026, 11:10 AM
    0 responses
    23 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    06-26-2026, 11:10 AM  
    Whole-Genome Sequencing Traces Faroe Islands Ancestry to a North Atlantic Founder Population by SEQadmin2
    Started by SEQadmin2, 06-17-2026, 06:09 AM
    0 responses
    55 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    06-17-2026, 06:09 AM  
    View All
    Working...