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Originally posted by id0
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We'll give that a try. Thanks! -
hi,Originally posted by id0 View PostMy original BWA issue did not get resolved. I am using Mac OS X 10.7 and I have seen reports of other Mac users reporting the same problem. When I switched from BWA 0.6.2 to BWA 0.5.9, it was working fine.
As I was writing this reply, I noticed BWA 0.7.0 was released last week. I am not sure if that one solves any of the Mac issues. See changelog here:
I used BWA for 2x250bp reads and I did not encounter any problems. From BWA main page:
It looks like the last 3 versions of bwa, including the latest released last week, don't work on a Mac. I downloaded 0.5.10 and I think things are working now. It's definitely taking much longer to make the .sai file, so I see that as a good sign.
Thanks!Comment
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I just ran into this after I aligned human paired-end sequences against the tiny non-human genome included in the distribution kit.
Opps:-(
BillComment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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