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Hello all! I need to analyze some RNAseq data in .wig (wiggle) format, that I obtained from a paper, but I am unfamiliar with this format. I want to filter for a certian set of genes (ovary related) and then sort based on differential expression. Have you had experience with .wig files? Can anyone point me in a helpfull direction?
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Information about the .wig format: https://genome.ucsc.edu/goldenPath/help/wiggle.html -
I found a post on BioStar that suggested using BEDOPS to convert to the more common BED file and proceed in analysis from there.Comment
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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