Dears colleagues,
Last year, I made the assembly of a complete bacterial genome using 454 sequencing reads. However, as you probably know, 454 technology is prone to frameshift. Consequently, I sequenced the strain DNA using Illumina MiSeq. Is there a software to correct reference genome (in fasta) with illumina reads? I know there are iCORN and iCORN 2, unfortunately I have experimented some problems with them.
Thank!
Antony
Last year, I made the assembly of a complete bacterial genome using 454 sequencing reads. However, as you probably know, 454 technology is prone to frameshift. Consequently, I sequenced the strain DNA using Illumina MiSeq. Is there a software to correct reference genome (in fasta) with illumina reads? I know there are iCORN and iCORN 2, unfortunately I have experimented some problems with them.
Thank!
Antony
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