Any suggestion on how to get to know the seq data is from which platform?

[arkilis]

Got some data from other lab people. They are not sure what platform it is. So I used the fastqc which can have a check on the read length and some tips on what platform it is. i.e. Quality scores across all bases (Sanger/ illumina 1.9 encoding). I would reckon it is probably from sanger.

Any other thoughts?

Thanks,

[Brian Bushnell]

Posting read headers may help; someone may recognized them. Also, a read length histogram (or range) might help narrow it down.

[arkilis]

Posting read headers may help; someone may recognized them. Also, a read length histogram (or range) might help narrow it down.

Thanks for answering.

One example line here is :

@M00474:102:000000000-A9000:1:1101:19225:1361 1:N:0:1
TGCTCACCTCGCCGCTGCACTGTGAAGCTCTCACCAACCCCGTAGTTGTTTCTGCACACGGTGTCCACCTGGCCCCGCCTGTCCTCCAGGATGTCCTTCTGGCTGTTCCAGGACTCGGCGACAGGCCGCCCTAGCTCCGTCACCGCCCGGTACTCCCCCCCGTCGCTGTCGAAGCGCACGAACTCCTCCTGGTTATAGAAGAGTCTTTCCAGGAACTGCACCCGCTCCGTCCCGTTGAAGAAATGACACTT
+
CACCCGGGGGBFGCGGGGFGGFGGDEGGFGGGGGGECFGGGGGDCFGFF,CFGGGGGGGD@FCGGGFGGGC,EFGGGGGG@FGGGGGFFFFGGFGGGGGG9<FGCFGGGGGFGFGGECFFDFB:BFGEFGGGDCFFGG@EGGGGGGGGG7E@EFGFGGG*CEEG:CEEGGGF5EDDGDEEGDGGGFFFGGFCFFFFF6D*)9CFGFFGFGG9:?(8?FF7?FB>>9>BB??FFFBFF()6<2:AABA7?B9

Any idea? Thanks again!

[Brian Bushnell]

Given that it's 251bp long, and the name look like the MiSeq convention, I would say that it's an Illumina MiSeq read.

[arkilis]

Given that it's 251bp long, and the name look like the MiSeq convention, I would say that it's an Illumina MiSeq read.

You are right. Mr. Genius! Just get confirmed with client. MiSeq.