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Hi, We are currently moving our amplicon sequencing library prep over to an automatic platform. Before outlaying for machinery and programming, can anyone advise of positive or negative experiences with the different platforms available. We currently have in house Epimotion 5075 and Hamilton Microlab StarLet. We would like to automate magnetic bead clean up for each of the PCR's and pooling/quantification. Currently, our first attempts to get the Hamilton up and running have been quite disappointing so I would like to know if it is worth persevering?
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...