Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Fernas
    Member
    • Apr 2013
    • 74

    #1

    Can Trimmomatic handle Colorspace Reads

    I have a RNASeq fastq file (first 4 lines are):
    =====
    @SRR179591.1.1 mendel_20110320_FRAG_BC_Ryan_RNA_Seq_2_58_235_F3 length=50
    T.33.2010.0313.1231222320.1.3312.0.12.0212.0.013000
    +SRR179591.1.1 mendel_20110320_FRAG_BC_Ryan_RNA_Seq_2_58_235_F3 length=50
    !!A+!@>A5!@59%!=A'6=4@5'A!9!55=1!(!0-!'%-%!&!%&%60'
    =====

    This fastq format is Color-space. I am wondering if Trimmomatic can handle such file by trimming reads based on quality and adapters?
    (I did not see in the documentation of Trimmomatic that it can handle Color-space reads).
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    You will need to use Cutadapt: https://code.google.com/p/cutadapt/

    Comment

    • Fernas
      Member
      • Apr 2013
      • 74

      #3
      Thank @GenoMax.
      Actually, I have 100 fastq files. 95 of them contain quality values encoded in Phred33 (so I processed them with Trimmomatic), while 5 files are Color-space. I wanted to use Trimmomatic for all the 100 files for simplicity but it seems that I need to process those 5 files with Cutadapt.

      Comment

      • GenoMax
        Senior Member
        • Feb 2008
        • 7142

        #4
        If you are worried about consistency then you could re-process them all with cutadapt.

        Comment

        Latest Articles

        Collapse

        • SEQadmin2
          Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
          by SEQadmin2



          CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

          Despite this, “CRISPR helped turn genome editing from a specialized technique into
          ...
          07-31-2026, 11:01 AM
        • SEQadmin2
          Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
          by SEQadmin2


          Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

          The systematic characterization of the human proteome has
          ...
          07-20-2026, 11:48 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by SEQadmin2, Today, 10:05 AM
        0 responses
        8 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 08-13-2026, 12:22 PM
        0 responses
        32 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 08-11-2026, 10:35 AM
        0 responses
        24 views
        0 reactions
        Last Post SEQadmin2  
        Started by SEQadmin2, 08-06-2026, 07:41 AM
        0 responses
        38 views
        0 reactions
        Last Post SEQadmin2  
        Working...