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Hiseq 4000
Hiseq 3000
Nextseq 550
HiseqX 5
Hiseq 3000
Nextseq 550
HiseqX 5
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Any idea if the HS4000/5000 are using 4-color or 2-color chemistry? -
I think that's Nextseq only?
Looks to me like they've just stuck HiseqX-style flowcells in the 2500 system.Comment
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Except that I'm pretty sure the X-series do use 2-dye chemistry, the same as NextSeq...Comment
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Possible upgrade path for current 2500 owners?Originally posted by GW_OK View Post
I am not going to hold my breath though.Comment
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Can't find any literature that says anything other than the Nextseq uses 2 colors. In fact, isn't this why the Nextseq currently has GC problems? Haven't heard anything about that with the HiseqX...Originally posted by Brian Bushnell View PostComment
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Originally posted by GenoMax View Post
LOL, just like I was able to upgrade my v2-camera 2500's for the v4 chemistry....Comment
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New toys for one person is a funding/migration headache for another. Especially if there is no upgrade path. :-(Comment
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Made the title more explicit and promoted to the front page...Comment
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This http://blog.illumina.com/blog/illumi...eq-4000-system says the 4000 uses 4-color.
This http://www.illumina.com/content/dam/...0-2014-057.pdf says that 1 flowcell does 50 transcriptomes per run at 50M reads each, so that is >300M reads per lane. Looks faster as well, if the longest run time is 3.5 days (not sure if that would be for 1x150bp or 2x150bp).Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.comComment
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There are data from PCR-free, RNA-Seq and NRC libraries sequenced on HiSeq 4000 in BaseSpace.Comment
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Heard from someone that the cluster and reagent kits are similar in price to current Hiseq 2000 reagents, which would mean the price per read is quite a bit lower.Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.comComment
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#SNPsaurus:
I assume it will be 2x 150 bp in 3.5 days.
2.1 to 2.5 billion clusters per flow cell, in a 2x150 bp run is 630 - 750 Gb.
With a speed >200Gb per day a 2x 150 bp run will take 3.5 days.
I wonder if we can upgrade the 2500 V4 machine to a 4000 version...Comment
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It looks to me that the 3000/4000 only accept 8 lane flow cells (high-output). If true then the trade off for throughput is flexibility.Comment
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Ditto. I think supporting the patterned flow-cells would require too much of a reworking of the guts...Originally posted by GenoMax View Post
Seems like they are following the Apple product release model...Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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