What would be the benefit from using 2 x 8bp dual indexes vs say... 1 x 16bp single index for a paired end read?
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Last edited by Ingeneious; 01-16-2015, 05:07 PM. -
One good reason is to identify barcode swapping between samples that can happen during PCR:
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How many adapters or primers would you need for each of those scenarios to identify a given number of samples?Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.comComment
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Thanks, this makes sense. According to the paper, it looks like contamination is also an issue, if you pool after PCR.Originally posted by atcghelix View PostComment
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I think SNPsaurus's point is more important, however. You can get by with far fewer distinct adapters by using the combinatorial aspects of a two-index system.Comment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
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