Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • kapr0007
    Junior Member
    • Oct 2014
    • 5

    adding RG tags to bam files

    Hello all,

    This is my first post and I'm new to bioinformatics
    I am trying to add RG tags and sample ID to a list of sorted bam files, i have tried all the above mentioned scripts. i was able to add the RG tags to all my files, later calling with freebayes for SNP, my ouput vcf file has only the header part
    #CHROM POS ID REF ALT QUAL FILTER INFO FORMAT L6-6

    the rest is empty. could some one help me with this, all i need is after i call for SNP, i would like to have all the genotypes labeled with the specific sample IDs.

    Thank you all
  • sarvidsson
    Senior Member
    • Jan 2015
    • 137

    #2
    See http://seqanswers.com/forums/showpos...4&postcount=36
    Last edited by sarvidsson; 02-23-2015, 03:49 AM. Reason: Double posting

    Comment

    Latest Articles

    Collapse

    • GATTACAT
      Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
      by GATTACAT
      Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
      07-01-2026, 11:43 AM
    • SEQadmin2
      Nine Things a Sample Prep Scientist Thinks About Before Sequencing
      by SEQadmin2


      I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

      Here are nine questions we think about, in roughly the order they matter, before...
      06-18-2026, 07:11 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 07-02-2026, 11:08 AM
    0 responses
    19 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-30-2026, 05:37 AM
    0 responses
    20 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-26-2026, 11:10 AM
    0 responses
    21 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-17-2026, 06:09 AM
    0 responses
    54 views
    0 reactions
    Last Post SEQadmin2  
    Working...