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  • zoe561
    Junior Member
    • Jul 2014
    • 1
    #1

    ATAC seq lysis time

    Hello,

    I was wondering if anyone has attempted the ATACseq protocol on primary mouse basal keratinocytes and has some idea of optimal lysis time for 50,000 cells?

    Thanks!
    Tags: None
  • Agun
    Junior Member
    • Jul 2013
    • 8
    #2
    Hello, did you find the answer you needed?

    I am also interested in ATAC-seq for our population of keratinocytes. Perhaps we can help eachother out.

    Comment

    • obischof
      Member
      • Jan 2011
      • 11
      #3
      Originally posted by zoe561 View Post
      Hello,

      I was wondering if anyone has attempted the ATACseq protocol on primary mouse basal keratinocytes and has some idea of optimal lysis time for 50,000 cells?

      Thanks!
      Has any of you two tried ATACseq yet and if yes do you see a nucleosomal-like pattern after tagmantation? In our hands we only see a smear.

      Comment

      • Agun
        Junior Member
        • Jul 2013
        • 8
        #4
        No, It seems a little bit complicated without having seen anybody do it.

        What is your experience?

        Comment

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