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I'll try to download all the files again maybe someone is corrupted
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I think I find the problem, I cannot find the source of the .fq files, I have it but I don't know how xd, what is the oficial source?Comment
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Based on the file names tophat should be working. Well try doing a fresh download in a new directory.Comment
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Get the data files from the GEO link: http://www.ncbi.nlm.nih.gov/geo/quer...i?acc=GSE32038 GSE32038_simulated_fastq_files.tar.gzOriginally posted by lupid View PostComment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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