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  • linda2015
    Junior Member
    • May 2015
    • 2
    #1

    barcode library for ion torrent

    Hi everybody, I am new in the NGS field. I want to ask a question. If I want to barcode my samples, should I do paired barcode or one side barcode, considering the reading length of ion torrent, If the fragment is more than 300bp, maybe there will be an issue to do paired barcod?

    I want to do a pilot experiment to test my propose. but I hardly find literature regarding to my question.

    I wonder if any of you have experience on this, can you share your thoughts?

    Greatly appreciated!
    Tags: barcode sequencing, ion torrent
    • maven
      Member
      • Oct 2009
      • 11
      #2
      One barcode because Ion Torrent software doesn't look for barcodes on the other ends.

        Comment

        • linda2015
          Junior Member
          • May 2015
          • 2
          #3
          Originally posted by maven View Post
          One barcode because Ion Torrent software doesn't look for barcodes on the other ends.
          thanks for your reply,
          Another question is, I am not sure in which end to put barcode, in the beads end, is it P1 adapter and the other is A adpater? If I want to add barcode for sucessful read through, which end is better.

          Thanks a lot

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