Authors recommend at least 6 replicates/condition in an RNA-seq experiment, and up to 12 in studies where identifying the majority of differentially expressed genes is critical. They recommend experiments with greater than 12 replicates should use DESeq, while those with fewer than 12 replicates should use edgeR.
-
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
