Tweet
The NextPrep-Mag™ cfDNA Isolation Kit is designed for extracting cell-free DNA (cfDNA) from human blood plasma or serum for NGS library prep using a scalable magnetic bead-based format. Typical input plasma volumes range from 0.6 mL – 4 mL, although amounts of plasma outside this range are also compatible with the kit. The cfDNA can be used for next generation sequencing and PCR analysis. Suitability of the DNA for use in NGS library construction has been verified using the Bioo Scientific NEXTflex™ Cell Free DNA-Seq Library Prep Kit for Illumina Sequencing and the NEXTflex Cell Free DNA Library Prep Kit for Ion Proton and Ion PGM Sequencing.
-
-
How is the yield and purity compared to QIAamp Circulating Nucleic Acid Kit? -
Hi Anne,
Yields and purity are similar. We have data posted on our website comparing the NEXTprep-Mag cfDNA Isolation Kit to the QIAamp Circulating Nucleic Acid Kit.Comment
-
Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
-
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
Comment