Squeezing the most out of Nextera XT...?

SDK

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Join Date: Jan 2014

Posts: 17
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Squeezing the most out of Nextera XT...?

07-15-2015, 11:50 AM

Hi All,

I'm wondering if anyone has experimented with pushing the limits of Nextera XT in order to get more samples out of a kit? I want to sequence a bunch (~150) of mitogenomes generated via long-range PCR. I was hoping to find a way to stretch the Nextera XT kit (and indices) to get the most bang for my buck. Since the samples are amplicons I think I should have enough "room" on one MiSeq run to collect all 150 mitogenomes at high depth...but don't want to have to pay for 150 Nextera XT libraries. Any ideas? Can I just run smaller reactions of the Nextera XT sample prep kit?

Thanks for any ideas.
Tags: amplicon, illumina, miseq, mitogenome, nextera
alec

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Join Date: Apr 2011

Posts: 18
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07-16-2015, 07:11 AM

Have a look at this paper: http://journals.plos.org/plosone/art...l.pone.0128036
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