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Have you looked at the text here: https://github.com/chrisamiller/read...n/readDepth.Rd
If that is not detailed enough you should email the author directly for additional help: https://github.com/chrisamiller
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Hi GenoMax,Originally posted by GenoMax View PostHave you looked at the text here: https://github.com/chrisamiller/read...n/readDepth.Rd
If that is not detailed enough you should email the author directly for additional help: https://github.com/chrisamiller
Yes, i saw this already and it's not detailed enough, at least for me..
I sent email to the author and waiting for his response..
I was sure that every package in R must have vignette/ or at least simple manual..
Thanks,
Pap
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How about genomecov (http://bedtools.readthedocs.org/en/l...genomecov.html) or coveragebed (http://bedtools.readthedocs.org/en/l...overage.html)? Those should work for what you are trying to do.
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ohh cool!Originally posted by GenoMax View PostHow about genomecov (http://bedtools.readthedocs.org/en/l...genomecov.html) or coveragebed (http://bedtools.readthedocs.org/en/l...overage.html)? Those should work for what you are trying to do.
Thanks!! I like bedtools!
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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Channel: Articles
07-01-2026, 11:43 AM -
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...-
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