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  • cmccabe
    Senior Member
    • Jul 2012
    • 355

    samtools bam2fastq command

    I have samtools installed to /usr/bin, it was installed using sudo -apt-get install samtools on ubuntu. Is the below command incorrect? Thanks .


    Code:
     bam2fastq -o IonXpress_009_150603.fq --no-unaligned IonXpress_009_150603.bam
    bam2fastq: command not found
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    bam2fastq is not part of new samtools (v.1.2) Peter's post below. It is not in v.0.1.19.

    Use bamtofastq from bedtools: http://bedtools.readthedocs.org/en/l...amtofastq.html

    Note: How well this will work with a file from Ion you will have to tell us.
    Last edited by GenoMax; 08-13-2015, 07:45 AM.

    Comment

    • maubp
      Peter (Biopython etc)
      • Jul 2009
      • 1544

      #3
      There is a BAM to FASTQ function within samtools, available via:

      Code:
      samtools bam2fq

      Comment

      • GenoMax
        Senior Member
        • Feb 2008
        • 7142

        #4
        @cmccabe: You will need new version of samtools (v.1.2) for the functionality Peter mentions above.

        Comment

        • cmccabe
          Senior Member
          • Jul 2012
          • 355

          #5
          Thank you very much

          Comment

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