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  • neokao
    Member
    • Mar 2015
    • 30

    Redundancy analysis (RDA) heatmap for 16s NGS results

    My lab has been working on bacterial 16S-based microbiome analysis in mouse gut and fecal samples. We are trying make figures like the attached pdf file.
    I have seen several figures (based on redundancy analysis (RDA)) like that.
    I could generate heatmaps myself but their figures all look similar and I think they must be generated from the exact same software. However could not find the exact software to make these figures in their methods or figure legends.

    Any suggestion? Thanks.
    Attached Files

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

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