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Old 09-05-2015, 05:17 AM   #20
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Location: Midwest

Join Date: Aug 2013
Posts: 8

Hi suzumar, if you apply any kind of coverage normalization (whether with khmer or bbnorm) prior to assembly, you won't be able to use those reads to calculate the coverage of the contigs. However, you can go back and use the unnormalized reads to calculate the contig coverage.

Re the science paper, I don't know what they actually did; if you can find the software and/or detailed instructions in their methods, I'd be happy to comment.
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