Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • JdevSEQnoob
    Junior Member
    • Sep 2015
    • 4

    #1

    I Have Variants and positions and need to make a GTF file. Help

    Hi,
    I'm very new to this and I'm working on my first project. I have a list of Variants and their location. I have been tasked with creating a GTF file of this data and then seeing where the data points land. I have searched and have a wealth of knowledge on what makes up the GTF file, why its used, blah blah. But I Still have no idea how to convert the limited amount of data (3 columns) of data that i have into the larger file format of GTF. Please advise. It'd be MUCH appreciated!

    The goal of this piece of the project is to use the coordinates of Variants to find their location in the annotation and then visualize it.
    Last edited by JdevSEQnoob; 09-25-2015, 03:34 AM.
  • dpryan
    Devon Ryan
    • Jul 2011
    • 3478

    #2
    Just skip making a GTF and use the VCF file that you presumably already have. You can directly use a VCF file with IGV for visualization or things like bedtools for whatever sorts of intersects you might find useful. It's quite likely that many of the tools that you want to use already support the format you have.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
      by SEQadmin2



      CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

      Despite this, “CRISPR helped turn genome editing from a specialized technique into
      ...
      07-31-2026, 11:01 AM
    • SEQadmin2
      Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
      by SEQadmin2


      Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

      The systematic characterization of the human proteome has
      ...
      07-20-2026, 11:48 AM
    • SEQadmin2
      Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
      by SEQadmin2



      Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
      ...
      07-09-2026, 11:10 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, Today, 10:13 AM
    0 responses
    10 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-31-2026, 02:55 AM
    0 responses
    21 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-24-2026, 12:17 PM
    0 responses
    19 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-23-2026, 11:41 AM
    0 responses
    17 views
    0 reactions
    Last Post SEQadmin2  
    Working...