Are there any sub $200 library prep services for RNAseq?

[Myrmex]

We are looking at doing RNAseq on around 36 samples we have extracted and want to outsource the library prep. I have shopped around a little and everything seems to be above $200. Does anyone know of any "economy" services that are a little less expensive. Thanks in advance.

[HTSlab]

Hi Myrmex,

We can come close: $220 for 24+ Stranded RNASeq libraries. We still do full QC on the starting RNA and use the Illumina prep kits, along with full QC and qPCR on the final library pool for optimal loading. Are you with University of Illinois Chicago?

[Myrmex]

Hi, thanks for getting back to me—we are at the Chicago Botanic Garden. Am I correct in understanding that the price is $220 per sample but you need at least 24 samples to get that price?

[HTSlab]

Hi. Yes, you are correct, each sample is $220 if you have at least 24 samples. If you are State of Illinois then overhead is only 10% additional.

I'm happy to continue discussions if you'd like to email me, thanks:

clwright at illinois dot edu

[kerplunk412]

It looks like you can get prep for $223 per sample with 8 or more samples at the University of Texas Genomic Sequencing and Analysis Facility. I have worked with them for over 3 years on various projects as a postdoc at UT and while at Bioo Scientific and they are top-notch.

Also, you may want to consider doing the preps yourself, or finding a lab that would collaborate to do the preps there. If you use a kit from a less expensive company than Illumina (Bioo Scientific, Kapa, NEB, and others), you can prepare your libraries for well under $100 each.

[MU Core]

Hello, our facility can provide 36 stranded mRNA preps for $196.56 per sample. You can visit our website to review our services and find information to contact core staff directly.

http://www.biotech.missouri.edu/dnacore/

[Myrmex]

Thanks for all the help guys! This is a little pricier then we thought it was going to be, so we are figuring out the funds...

[kerplunk412]

Are you set up for molecular biology? I know it sounds intimidating, but library prep is really not that difficult, and you can save a lot of money doing it yourself. Depending on the facility, you may also get faster turnaround time preparing them yourself.

[LOH]

If you have a lot of samples (>24), which is in good quality and easy to handle, you can negotiate for some discount, for example, libraries for less than $200. However, if you are handling challenging samples, such as FFPE, degraded, low input, you can hardly get such a price. We usually offer a generous discount for new customers. Check here for our promotional price for RNA Sequencing.

[Genohub]

You can do a mRNA-seq library prep search on Genohub.com: https://genohub.com/#q=a9ce1f402a. Costs (including QC) start at $165/sample (no sample number minimums).

Each service comes with a quality and turnaround time guarantee