Does anyone use Fluidigm C1 instrument on cells using DNASeq script and final library prep for NGS? Our lab has followed their published protocol using a control cancer cell line & we haven't got good alignment of reads across all chromosomes and have run into some other technical issues that makes us think the WGA used on the C1 isn't that good. Part of the problem is that nearly all the published papers use C1 for RNA-Seq while there are very few DNA-Seq papers out there.
-
-
Every WGA method isn't that good. -
Do you use the C1 NextGenSeq? Fluidigm gives the illustra WGA kit as their choice. When using that, it doesn't work to evenly amplify the template. Does anyone use another kit than the one Fluidigm recommends in their protocol or another process that would be better to perform DNA Seq on single cells?Comment
-
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Today, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
Comment