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Hi everybody,
Currently, I am using Ion PGM Ampliseq Cancer Panel Hotspot v2 and Torrent Variant Caller.
Usually I have only few number of coverage, for example 30 of 200base reads. Sometimes this kind of data will not appear in TVC. I was wondering if the coverage of my data is too low or do I set the stringency too high.
I always set the variant frequency to "somatic" but I am little confused because in the user guide said that for somatic workflows the threshold is set to 4% frequency for SNPs and 20% for indels. But as you can see in the picture, the minimum variant allele is 0.02 (is it equal to 20% or 2%?)
upload img
Somatic:
image hosting websites
Germline:
photo sharing sites
Thank you for your attention, your help is much appreciated.
Currently, I am using Ion PGM Ampliseq Cancer Panel Hotspot v2 and Torrent Variant Caller.
Usually I have only few number of coverage, for example 30 of 200base reads. Sometimes this kind of data will not appear in TVC. I was wondering if the coverage of my data is too low or do I set the stringency too high.
I always set the variant frequency to "somatic" but I am little confused because in the user guide said that for somatic workflows the threshold is set to 4% frequency for SNPs and 20% for indels. But as you can see in the picture, the minimum variant allele is 0.02 (is it equal to 20% or 2%?)
upload img
Somatic:
image hosting websites
Germline:
photo sharing sites
Thank you for your attention, your help is much appreciated.