Like many folks working on low diversity libraries, we have been hit hard by the problems associated with the V3 kit - lots of time and effort wasted. Is this problem also common for high diversity libraries? I would appreciate any insights.
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I've had problems with high-diversity (gDNA) libraries with the 600-cycle V3 kits, even with a cluster density of 1K. Read 2 just tanked after about 50 bp. We are advising people not to run them at all until Illumina sorts out whatever problem this is. -
Thank you! Will it be possible to mix our MiSeq libraries with our HiSeq libraries (e.g., RNA-Seq, have not started yet) and running the mix libraries with HiSeq2500 or 3000?Comment
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I've run libraries on the MiSeq (usually for troubleshooting purposes) and then loaded them on a HiSeq once I was sure everything was okay with them. The loading concentrations are usually different, in my experience, but that should be the only issue you'll need to address.Comment
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Good to know-thanks!Comment
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
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