Questions on Agilent SureSelect Indexing Kit

if1

Junior Member

Join Date: Nov 2009

Posts: 2
- Share
- Tweet
#1

Questions on Agilent SureSelect Indexing Kit

05-17-2010, 08:14 AM

Hi,

does anybody have details on the new SureSelect Indexing Kits?
I am interested in targeting 12 samples and pool and sequence them on 1 lane of Illumina GAII.

My question is:

- What is the maximum amount of DNA that I can target using a 55k baits SureSelect library? For one sample with a Paired-End approach one can target up to 6.6Mb. Do I have to divide this amount by the number of samples that I want to target (i.e. 6.6/12=0.55Mb max per sample)?

Thanks!
if1

Last edited by if1; 05-17-2010, 08:16 AM. Reason: typo
Tags: illumina sequencing, multiplexing, target enrichment
aranarh

Junior Member

Join Date: May 2010

Posts: 1
- Share
- Tweet
#2

05-18-2010, 06:32 AM

IMHO, indexing do not influence the amount of DNA you can capture with one SureSelect library. There is still one library for the enrichment of one DNA sample. Usage of indexing adapters and primers only allows you to sequence up to 12 enriched samples on a single GAII lane. Unfortunately it do not allow you to enrich 12 samples using a single SureSelect library...
Comment

Previous template Next

Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems

by SEQadmin2

Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
...
- Channel: Articles
07-09-2026, 11:10 AM
Cancer Drug Resistance: The Lingering Barrier to Rising Survival

by SEQadmin2

Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.

There is no single reason why many patients don’t respond to treatment as expected. Cancer is...
- Channel: Articles
07-08-2026, 05:17 AM
Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing

by GATTACAT

Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
- Channel: Articles
07-01-2026, 11:43 AM

Topics	Statistics	Last Post
UC San Diego Bioengineers Map Gene Function in Human Stem Cells by SEQadmin2 Started by SEQadmin2, 07-13-2026, 10:26 AM	0 responses 18 views 0 reactions	Last Post by SEQadmin2 07-13-2026, 10:26 AM
New Analysis Splits Leukemia Into 16 Epigenomic Subgroups by SEQadmin2 Started by SEQadmin2, 07-09-2026, 10:04 AM	0 responses 30 views 0 reactions	Last Post by SEQadmin2 07-09-2026, 10:04 AM
Genome-Wide CRISPR Screen Uncovers Unlikely Psoriasis Target by SEQadmin2 Started by SEQadmin2, 07-08-2026, 10:08 AM	0 responses 16 views 0 reactions	Last Post by SEQadmin2 07-08-2026, 10:08 AM
Engineered Protein Motor Takes Its First Steps Along DNA Track by SEQadmin2 Started by SEQadmin2, 07-07-2026, 11:05 AM	0 responses 34 views 0 reactions	Last Post by SEQadmin2 07-07-2026, 11:05 AM

Unconfigured Ad

Questions on Agilent SureSelect Indexing Kit

Comment

Latest Articles

ad_right_rmr

News