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  • Ghada
    Junior Member
    • Nov 2014
    • 5
    #1

    3500 genetic analyzer low quality value

    Dear professor
    we have a new 3500 genetic analyzer that sanger/dye terminator from applied biosystem. All amplicon have e low to medium quality value the same site 75-85 bp including the control PGEM, however the seq standard is ok. please check attached files
    Any comments
    regards
    Ghad
    Attached Files
    Tags: None
  • MU Core
    Member
    • Apr 2008
    • 60
    #2
    Ghada, you're observing the presence of "dye blobs" which is the result of poor removal of unincorporated dye terminators from the post-sequencing reaction. A common issue in dye terminator sequencing. You'll want to revisit your reaction clean-up method to see if changes can be made to optimize the method. Or, you may want to consider a different approach to reaction clean-up. Performing a google search with "removing dye blobs" will provide you with information on the topic.

    Comment

    • Ghada
      Junior Member
      • Nov 2014
      • 5
      #3
      thank you MU core for your help.
      Regards
      Ghada

      Comment

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