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Old 03-05-2016, 08:28 PM   #28
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Location: perth

Join Date: Mar 2016
Posts: 2
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Hi Brian,

I am using bbnorm.sh to filter low-depth reads and correct them together. I have a question about it as below:
The raw pair-end reads file size(.gz): 223+229Mb;
After running bbnorm.sh:111+113+64Mb (out, out2 and outt);
So, what is different between 64Mb reads with filtered reads(164Mb=223+229-111-113-64).
This is my running shell:
bbnorm.sh in=input_fq1.gz in2=input_fq2.gz zerobin=t prefilter=t maxdepth=1000 lowbindepth=10 highbindepth=500 ecc=t out1=bbnorm.fq1.gz out2=bbnorm.fq2.gz outt=exclued.fq.gz

Thanks,
Xiao
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