Tweet
I have been asked a few times and have not been able to find a clear answer online. Is there any reason that RNA pellets cannot be shipped at room temp in 100% isopropanol?
-
-
isopropanol is a flammable, but you would need to check with your shipper if the volumes you are using are okay for airmail. -
We do recommend our customers from South America (shipments can be delayed quite often) to ship their RNA after samples after ethanol precipitation, pellets washed twice with 70% ethanol, and then covered with a minimum amount of 80% ethanol (20ul).
We do not have any problems sequencing these samples.
Lutz
Comment
-
80% ethanol
Thanks to both of you?
These are shipped at room temp?
Is methylnick's concern about shipping a flammable a concern?Comment
-
Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
-
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
Comment