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Old 04-21-2016, 01:03 PM   #39
evanname
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Location: US

Join Date: Jan 2016
Posts: 1
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Brian, thank you so much for the excellent tools!

Is it possible to say at what level the error correction would be able to distinguish between sequencing errors and heterogeneity in the source sample?

For example, if the source was a 500bp PCR product and 2% of the molecules had a substitution at base 100, would BBnorm flag that as an error? Is there an approximate percent heterogeneity at any particular base that serves as the dividing line between 'error' and 'SNP'?

Thanks!
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