Homemade SPRI beads- hydrophobic or hydrophilic beads?

[JBKri]

We want to make our own Ampure beads for DNA cleanup and size selection.
We think we need the Sera-Mag Carboxylate-Modified SpeedBeads, but they come in two types; hydrophilic and hydrophobic. We are thinking the hydrophilic is what we need, but here they link to the hydrophobic variety.

[Don Stephano]

Hi,

I'm using Fisher #09-981-123 (GE Life Science 65152105050250) according to the protocol posted by Ethan-omics: https://ethanomics.wordpress.com/201...pure-xp-beads/

No problems so far, the selfmade beads are working perfectly.

[jwfoley]

Hi,

I'm using Fisher #09-981-123 (GE Life Science 65152105050250) according to the protocol posted by Ethan-omics

Those are the same ones in the protocol JBKri is looking at, since we just copied the purchasing information when we improved on the other aspects of Faircloth & Glenn's protocol.

JBKri, why do you think they should be hydrophilic beads? Or, why do you think the carboxyl coating is hydrophobic?

[JBKri]

Those are the same ones in the protocol JBKri is looking at, since we just copied the purchasing information when we improved on the other aspects of Faircloth & Glenn's protocol.

JBKri, why do you think they should be hydrophilic beads? Or, why do you think the carboxyl coating is hydrophobic?

Thank you both! A colleague uses the homemade beads, and at one point there was some confusion about the beads; we were mistakenly given a product code for hydrophilic beads.