[nucacidhunter]

I wonder if anyone have update on Sequel performance and how it compares to RSII.

[GenoMax]

There was a PacBio user group meeting in Baltimore (in last two weeks) where an update was given about Sequel performance (based on a note I saw from a FAS). Apparently that information has not been published but if someone on SeqAnswers was in attendance then they may be able to comment.

[nucacidhunter]

Thanks GenoMax. The system release was announced almost 8 months ago and I thought there might be some installed machines in production or in validation phase where some operators might have been trained and seen some real data to compare with Sequel announced specs or RSII.

[AllSeq]

Thanks GenoMax. The system release was announced almost 8 months ago and I thought there might be some installed machines in production or in validation phase where some operators might have been trained and seen some real data to compare with Sequel announced specs or RSII.

We don't have any data, but we have a (growing) list of service providers who have installed the Sequel platform: http://ngs.is/PBSequel

[nucacidhunter]

They do not seem to offer any service on the Sequel.

[GenoMax]

Thanks GenoMax. The system release was announced almost 8 months ago and I thought there might be some installed machines in production or in validation phase where some operators might have been trained and seen some real data to compare with Sequel announced specs or RSII.

This is a big mystery. There are reports of machines in the wild (at least with close collaborators of PacBio) but no one seems to have released real data as yet. There is nothing EBI-ENA at this time either.

[Brian Bushnell]

We have two of them, but they are still being qualified.

[spore]

A few service providers had told me they expect to start providing Sequel services in June. I expect we should see some data soon?

At the UGM pacbio did mention they expect to start shipping their new chip at the end of the month along with a new loading protocol. So maybe service providers are waiting for that? I'm sure Sequel owners here would have a better idea of whats going on though.

[luc]

Hi Brian,
is this with your previous or the new employer?
Thanks!

We have two of them, but they are still being qualified.

A Sequel talk from April: https://www.youtube.com/watch?v=hmx3HGs9ims

[Brian Bushnell]

Hi Brian,
is this with your previous or the new employer?
Thanks!

Both! I left Google and came back to JGI. So, it's at JGI.

[GenoMax]

Both! I left Google and came back to JGI. So, it's at JGI.

Google's loss but good for us

We will have to count on you @Brian to see some unbiased eval of Sequel data once you get your hands on some.

[luc]

Google's loss but good for us

Ditto!


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[spore]

Does anyone have information on how the Sequel data compares to the RSII? And when service providers will begin offering Sequel services?

[GenoMax]

It is hard to tell but the description for this dataset seems to include verbiage about Sequel (not sure why the submitters would include that if this was a RSII): http://www.ebi.ac.uk/ena/data/view/SRX1684788

Most likely a red herring but who knows. Raw bax/bas.h5 files are available in case someone is curious (http://trace.ncbi.nlm.nih.gov/Traces...run=SRR3342814 under Download tab).

[sajeshpk]

No... most of what we sequence comes from remote users. But, we do all the library construction here, and I believe we reject samples that do not have high molecular weight DNA, or insufficient mass.

Hi
We are facing some issues with our new sequal system installed recently. Pacbio team is unable suggest loading clear loading concentration of different sized library.
The out put varies with each run and the maximum we got till now is 3.2gb for 2 kb library.
For the higher sized library the runs will yield 0.2-0.9gb per SMRT.We are unable to find out where the problem lies.Also after completing each run the volume of libraries pickedup from the sample well is not consistent.
Pac bio suggestion for the loading conc is 50-60pM for any size library.

There is another suggestion from PacBio is that system and smrtlink need to be restarted every 24 days, Is it true?

Please help