Tweet
Hi all, while looking for SNPs for virus quasispecies in a badnavirus using RNA that was gel-excised with maximum length of 100, maybe 200 bp I find definitive read alignment hotspots to the master genome in all 3 samples. generally people have been finding these hotspots in the populations of siRNA of 21,22 or 24 nt length, products of silencing. however the hotspots I find are in the slightly longer reads. Sequencing ran for 36 cycles, so they are 36 nt long. Also, 90+% of these reads with potential lengths between 30 and 100 nt are aligning to the sense strand. I am trying to come up with an explanation for these two things and drawing a blank. I am not a virologist or even microbiologist, so am I missing something obvious? I apologize if this is not the place to ask this question, and would appreciate any answers or other places to ask this question.
-
Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.
The systematic characterization of the human proteome has...07-20-2026, 11:48 AM -
Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
...07-09-2026, 11:10 AM -
Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
There is no single reason why many patients don’t respond to treatment as expected. Cancer is...07-08-2026, 05:17 AM