Reusing/Recycling Probes?

[naji]

Hi all,

Just wondering if anyone has had any luck with successfully, or trying to, recycle/reuse DNA probes? Any advice, ideas, or insights would help.

Thanks!

[GenoMax]

Why would you want to do that? This (to a non-bench scientist) sounds like a bad idea.

[naji]

I figure to save money on probes, but also, if this is something that can be done (relatively) "easily" and efficiently, it could allow for using recycled probes as a control variable for assay development?

[nucacidhunter]

Do you mean biotinylated sequence capture probes?

[HESmith]

Why would you want to do that? This (to a non-bench scientist) sounds like a bad idea.

This (to a bench scientist) also sounds like a bad idea. In theory, you can strip the sequence capture probes. In practice, you'll get contaminating sequences from previous captures.

[BioDynami]

Compared to the money you saved, you will get more trouble in terms of library contamination and possible bias of probe contents.

[Xploration]

What kind of probes are you asking about? I re-use DIG-labeled PCR product probes for Southern blots.