[thermophile]

I'm not surprised that they moved to subscription. But an annual $5000 buy in to give someone the opportunity to pay to use apps is ridiculous.

[GenoMax]

I'm not surprised that they moved to subscription. But an annual $5000 buy in to give someone the opportunity to pay to use apps is ridiculous.

To be fair, that $5k (if that is what the cost of the mid-tier subscription is) appears to include two additional features.

Whether "Multi-user access" and 8hr of bioinformatics professional services support is worth $5K, is open to debate If you don't have any local IT infrastructure then that may be your sole option.

[kcchan]

- The newest version of bcl2fastq2 will now treat N bases properly (as 'wildcards' so-to-speak)

That issue still hasn't been fixed as of the the most recent 2.18 update.

[GenoMax]

That issue still hasn't been fixed as of the the most recent 2.18 update.

Software release note for 2.18 has this listed as "known issue"

“N” is incorrectly allowed as an index sequence character in the sample sheet. When used, this will cause a mismatch for any sequence character other than “N”.

[Jessica_L]

I would imagine some of the third party developers aren't too happy either since their apps are now stuck behind a paywall.

Does that apply to all apps or just the third party ones that we would normally be paying for? Right now, it looks like the usual basic analyses I'm used to running are still accessible from a basic account, but I think my account is still on a trial basis from the changeover.

[thermophile]

I can select a sample, launch FastQC or 16S metagenomics, but then I don't have the start button

[kcchan]

There is a message where the "Launch App" button used to be:

To use this app, upgrade to a Professional subscription

This includes all published apps as well as any custom apps you have in your developer account.

[Jessica_L]

I can select a sample, launch FastQC or 16S metagenomics, but then I don't have the start button

Wait, so we can't even do FastQC with a free account? I was able to run it, but like I said yesterday, my account is still in trial mode. Are you just trying to use the Illumina/BaseSpace Labs 16s metagenomics app? I'm not hopeful for my Amplicon DS analyses, then....

[GenoMax]

Are the "free" accounts only meant for people who have one (or a small number of) Mini/Mi/NextSeq and need to do basic distribution/sharing of data? i.e. small core facilities.

[Jessica_L]

That's kind of what I'm wondering, too, GenoMax? We have a pretty small operation, but I do also send out for larger jobs and I still need to analyze data sometimes.

[thermophile]

Jessica, I can't run any apps at all. My understanding is that every new account gets a 30day trial so I guess you could just make a new email and account every month to continue using the apps.

[GenoMax]

Jessica, I can't run any apps at all. My understanding is that every new account gets a 30day trial so I guess you could just make a new email and account every month to continue using the apps.

While that may work short term, it may land you on Illumina's blacklist (if they watch for what IP's the accounts are logging in from).

Small cores may need to adjust their prices (and account for $5K subscription) if they find BaseSpace indispensable for daily business.

[Jessica_L]

That's good to know, thermophile, thanks. I'm probably going to have to ask for that $5k to be added into our budget for next year.

[thermophile]

While that may work short term, it may land you on Illumina's blacklist (if they watch for what IP's the accounts are logging in from).

Small cores may need to adjust their prices (and account for $5K subscription) if they find BaseSpace indispensable for daily business.

True. I'm likely going to go back to ignoring the basespace apps and use it just for fastq distribution which is still currently feasible for my miseq only core on a free account.