edger and ccle

[hibari]

Hi all,

This is my first post here, so I am taking the opportunity to thank all the people who make this forum live.


It is possible to find read counts from CCLE here :

https://ocg.cancer.gov/ctd2-data-project/translational-genomics-research-institute-quantified-cancer-cell-line-encyclopedia

I would like to use these data to find genes involved in resistance to treatment.
I would like two group these cell lines in "sensitive" and "resistance" and test for the DEGs.
However there is no technical replicats for these data.

Let's say I have 32 cell lines in the "resistant" group and 32 cell lines in the "sensitive" group.
Can I perform a DEG analysis with edgeR ?
Can the 32 cell lines in each group be considered as replicats ?
Do you think my approach is valid ?

Thank you very much.