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  • Niels Wagemaker
    Junior Member
    • Mar 2016
    • 6
    #1

    epiGBS

    Nature methods epiGBS article:

    Please notice our Nature methods epiGBS article on epiGBS:
    epiGBS: reference-free reduced representation bisulfite sequencing
    doi:10.1038/nmeth.3763

    I made this thread to help get you information about new updates on the protocols (new enzyme combinations, adapter innovations) and to get in contact for help or collaborations.

    Our great protocol will really help you to steam up your DNA genotyping and methylation research on big amount of samples for affordable costs.

    Niels Wagemaker
    Tags: epigbs, gbs, methylation, smp, snp
  • ecSeq Bioinformatics
    Senior Member
    • May 2012
    • 492
    #2
    DNA Methylation Data Analysis Workshop
    How to use bisulfite-treated sequencing to study DNA methylation

    When?
    22. - 25. November 2016

    Where?
    Leipzig, Germany

    Link?
    404 Not Found
    http://www.ecseq.com/workshops/workshop_2016-07-NGS-DNA-Methylation-Data-Analysis


    ecSeq Bioinformatics is Europe’s leading provider of hands-on bioinformatics workshops and professional data analysis in the field of Next-Generation Sequencing (NGS).

    Comment

    • Niels Wagemaker
      Junior Member
      • Mar 2016
      • 6
      #3
      Epigbs enzyme combinations

      Next to the published enzyme combinations we have available:

      Improved solutions for

      Smal genomes: (arabidopsis): csp6I * NsiI
      Average genomes: (scabiosa columbaria): AseI * NsiI
      Large genomes: (sequoia): PacI *NsiI

      All with methylation insensitive enzymen.
      PacI needs different adapter set as other 2 enzyme combi's

      More info: mail me

      Comment

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